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Analysis of cost, time, and accuracy of GFP sequences assembled from oligos produced via enzymatic (EDS) or chemical (CDS) DNA synthesis.

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Assessment of enzymatically synthesized DNA for gene assembly

doi: 10.3389/fbioe.2023.1208784

Figure Lengend Snippet: Analysis of cost, time, and accuracy of GFP sequences assembled from oligos produced via enzymatic (EDS) or chemical (CDS) DNA synthesis.

Article Snippet: Phosphoramidite solid-phase chemical DNA synthesis (CDS) ( ) is the standard method for producing de novo single-stranded DNA (ssDNA) and is used on a large scale by commercial vendors to produce oligonucleotides (oligos) for PCR, cloning, and increasingly for de novo construction of genes and plasmids ( ; ).

Techniques: Produced